Skip to main content
Figure 5 | Journal of Inflammation

Figure 5

From: miRNA-146 negatively regulates the production of pro-inflammatory cytokines via NF-κB signalling in human gingival fibroblasts

Figure 5

NF-κB subunits directly bind to the miR-146 promoter. We cloned the miR-146 2 kb promoter region into the 5’ site of the luc2 reporter gene on the pGL4 plasmid. We co-transfected 200 ng of the reporter plasmid, 20 ng pRL-TK-Renilla-luciferase and 100 ng of p50/p65 pcDNA3.0 plasmid into the HGFs. Luciferase activity was measured using the Dual-Luciferase Reporter Assay System according to the manufacturer’s instructions. The expression levels are presented as the mean ± SD. *p <0.05 versus mock control.

Back to article page