Figure 2

miRNA-146 expression is down-regulated when IRAK4 and NF-κB signals are blocked. The indicated amounts of TPCA-1 (an IKK-2 inhibitor), PD98059 (a MEK-1/2 inhibitor), IRAK1/4 inhibitor and SP600125 (a JNK-1/2 inhibitor) were added to the HGF cultures with or without P.g LPS (1 μg/ml) stimulation for 24 h. Next, total RNA was purified, and the miR-146a (A) and miR-146b (B) levels were analysed via real-time PCR. The results are expressed as the mean ± SD of three independent experiments. *p <0.05 versus P.g LPS-treated no-activation controls.