Figure 1

Multiple kinases are induced in P.g LPS-treated HGFs. HGFs from donors were cultured for at least 4 passages. After stimulating with P.g LPS (concentration 1 μg/ml) for different indicated time, HGFs were counted and harvested, and phosphorylation and expression levels of TLR4 downstream kinases such as IRAK4 (A), Erk1/2 (B), JNK(C), and IκB-α (D) were analysed by monoclonal and polyclonal antibodies. The concentrations of TPCA-1 (an IKK-2 inhibitor), PD98059 (a MEK-1/2 inhibitor), and SP600125 (a JNK-1/2 inhibitor) are all 1 μM. (E) Quantitative assay of (A)-(D), data are shown as mean ± SD of three independent experiments.