Figure 2

Augmentation of adenosine-mediated activation of the human A _2a R (hA _2a R) . (A) AEA061 enhances adenosine-mediated cAMP production by the hA_2aR. CHO-hA_2aR cells were incubated with adenosine, and AEA061 for 15 min. Mean cAMP levels ± SEM of a representative experiment is shown (n = 3). (B) AEA061 potentiates both the affinity and the efficacy of adenosine at the hA_2aR. CHO-A_2aR cells were incubated with AEA061 and varying concentrations of adenosine for 15 min. Mean EC₅₀ values of three experiments with SEM were 4.4 ± 1.3 nM and 1.5 ± 0.49 nM without and with AEA061 respectively. Increase in E_max by AEA061 = 2.1 ± 0.27 fold.