Figure 7

Triple labeling of ICAM-1, GFAP and DAPI and double labeling of ICAM-1 and NeuN in the frontal cortex after 6 days of reperfusion in normo- and hyperglycemic animals. (A-C) No colocalization of ICAM-1 with astrocytes; however, ischemia led to an increased number of GFAP positive astrocytes. (D) Occasional ICAM-1 positively stained neurons were detected in the sham control animals. (E) Ischemia increased the number of ICAM-1 positively stained neurons. (F) Diabetic hyperglycemia further increased the number of ICAM-1 positive neurons in the frontal cortex. (G) Bar graph summarizes the average number of neurons positively labeled by anti-ICAM1 antibody. *P <0.05 vs. sham group; #P < 0.05 vs. normoglycemic group at an identical reperfusion stage. Green color, ICAM-1; blue color, DAPI; red color, GFAP in A-C and NeuN in D-F. Magnification, 400X.