Figure 5
From: Upregulation of ICAM-1 in diabetic rats after transient forebrain ischemia and reperfusion injury
ICAM-1 mRNA in situ hybridization. Images were taken of the frontal cortex. (A,D) Negatively stained brain sections from sham controls. (B,E) ICAM-1 mRNA positive stained cells detected at 3 and 6 days of reperfusion in normoglycemic animals. (C,F) ICAM-1 mRNA positive stained cells in diabetic animals. (G) Bar graph summarizes the mean optical density of ICAM-1 mRNA in samples collected from the frontal cortex. Arrows indicate ICAM-1 positive neurons and arrow heads indicate ICAM-1 negative neurons. *P <0.05 vs. sham group; #P < 0.05 vs. normoglycemic group at an identical reperfusion stage. I/R, ischemia and reperfusion. DAB visualization, weak haematoxylin counterstaining. Magnification, 400X, bar = 20 μm.