Figure 5

Neutrophil infiltration occurred following ischaemia but was unaffected by AC administration. Renal IRI was induced in 8-week old male Balb/c mice by a right nephrectomy and ischaemia induced by occluding the left renal pedicle for 20 (mild ischaemia), 24 (moderate) or 25 mins (severe). Sham mice underwent a laparotomy only. Either PBS or 20×10⁶ early or late ACs were administered 24 hr prior to renal IRI. Mice were sacrificed 24 hr following IRI. (A) Representative images of the outer stripe of the outer medulla in non-injured and ischaemic kidney sections following Gr1 staining. In ischaemic kidneys neutrophil infiltration was observed in areas of necrosis. (Magnification: ×200; Scale Bar: 50 μM) (B) Scoring of Gr1 staining (expressed as Gr1⁺ area) demonstrates that neutrophil infiltration remained similar between PBS and AC treated mice with severe, moderate and mild ischaemic injury. Grey circle symbol = Non-injured kidney Grey square symbol = Ischaemic kidney. Data expressed as mean ± SEM and analysed by two-way ANOVA. ns = non-significant. Sham (n = 4), PBS (n = 7-8), AC (n = 8).