Figure 4

Microvascular congestion remained unaffected following AC administration. Renal IRI was induced in 8-week old male Balb/c mice by a right nephrectomy and ischaemia induced by occluding the left renal pedicle for 20 (mild ischaemia), 24 (moderate) or 25 mins (severe). Sham mice underwent a laparotomy only. Either PBS or 20×10⁶ early or late ACs were administered 24 hr prior to renal IRI. Mice were sacrificed 24 hr following IRI. A & C) Representative images of the outer stripe of the outer medulla in non-injured and ischaemic kidney sections following platelet (CD41) immunohistochemistry (A) and fibrin immunofluorescence (C) staining. In ischaemic kidneys microvascular congestion was observed as indicated by platelet and fibrin deposition. (CD41 - Magnification: ×200 & Scale Bar: 50 μM; Fibrin - Magnification: ×400 & Scale Bar: 25 μM) B & D) Scoring of platelet (B) and fibrin (D) staining (expressed as platelet/fibrin⁺ area) demonstrates that microvascular congestion remained similar between PBS and AC treated mice with severe, moderate and mild ischaemic injury. Grey circle symbol = Non-injured kidney Grey square symbol = Ischaemic kidney. Data expressed as mean ± SEM and analysed by two-way ANOVA. ns = non-significant. Sham (n = 4), PBS (n = 7-8), AC (n = 8).