Figure 5
From: P2X7 receptors are a potential novel target for anti-glioma therapies
Microglia from human glioma at 3 days post culturing showed P2X7R pore activity, as evidenced by dye uptake experiments. YOPRO-1 nuclear intensity was measured as an index of P2X7R pore capacity (n = 42 microglia being isolectin GS-IB4 positive from 3 human gliomas). This pore capacity was reduced with inhibition with a specific and irreversible antagonist of P2X7R, oxATP (250 μM of oxATP for 3 hours prior to YOPRO-1 incubation, n = 28 microglia, being isolectin GS-IB4 positive from 3 human gliomas).