Figure 6
HPLC chromatogram of standard mixture (A) and C. officinalis (B) at 240 nm. Morroniside (1), loganin (2). C. officinalis and 2 standards were subjected to HPLC analysis. An Optimpak C18 (4.6 × 250 mm) column was eluted with water and acetonitrile (86:14) at flow rate of 1.0 mL/min. The HPLC chromatogram is shown for the ursolic acid standard (C) and C. officinalis (D) at 200 nm. C. officinalis and ursolic acid were subjected to HPLC analysis. The Optimpak C18 (4.6 × 250 mm) column was eluted with water and acetonitrile (10:90) at flow rate of 1.0 mL/min.