Figure 3
From: Induction of intestinal pro-inflammatory immune responses by lipoteichoic acid
Activation of mesenteric DCs by L. acidophilus strains. (a-b) Single cells were derived from mesenteric lymph nodes (MLN) or spleens of the mice (n = 5/group) treated with NCK2031, NCK56, NCK2025, or PBS. Cells were stained with anti-CD11c, CD11b, CD103, MHC II, CD19, F4/80, IL-12, or TNFα antibodies. Live CD11c+ cells that were F4/80-CD19- were analyzed for their cytokine expression and expression of surface molecules (i.e., MHC II) by FACSCanto II. Experiments were repeated at least three times with similar outcomes.