Figure 4

Western blot analysis of STAT6 and phospho-STAT6 expression in bronchial mucosal biopsies of normal (N) and asthmatic (As) subjects. Representative Western blot analyses of experiments from 3 individual subjects in each group are shown (A). Actin expression is used to control for protein loading. Graphical representation of the data in (A) is shown in (B). (C) Failure to detect phospho-STAT6 (pSTAT6) protein expression in bronchial biopsies isolated from As and N subjects. pSTAT6 expression in control IL-4 stimulated HeLa cells confirms the ability of the antibody to detect pSTAT6. Each circle represents one subject. Horizontal bars represent median values.