Figure 1

STAT6 protein expression in peripheral blood T-lymphocytes isolated from asthmatic (As) and normal (N) subjects. Representative Western blot analyses of experiments from 3 individual subjects in each group are shown (A). Actin expression is used to control for protein loading. Graphical representation of the data in (A) is shown in (B). (C) Failure to detect phospho-STAT6 (pSTAT6) protein expression in peripheral blood T-lymphocytes isolated from As and N subjects. pSTAT6 expression in control Th2 cells confirms the ability of the antibody to detect pSTAT6. Each circle represents one subject. Horizontal bars represent median values.