Table 2 Antibodies used in this study
Primary antibodies | ||||
|---|---|---|---|---|
Protein/Antibody | Clonality* |    Supplied by | Epitope, location | Dilution |
STAT1 (sc-346) | Poly | Santa Cruz Biotechnology, Santa Cruz, CA, USA | raised peptide mapping near the C-terminus of STAT1 p84/p91 of human origin | 1 : 500 |
pSTAT1 (#9167) | Mono (r) | Cell Signaling, Technology, Danvers, MA, USA | phospho-STAT1 (Tyr701) (58D6), raised against synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human STAT1 | 1 : 1,000 |
STAT3 (#4904) | Mono | Cell Signaling | raised a STAT3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3 protein | 1 : 1,000 |
pSTAT3 (#9134) | Poly | Cell Signaling | phospho-STAT3 (Ser727), raised against a synthetic phosphopeptide corresponding to residues surrounding Ser727 of mouse Stat3 | 1 : 1,000 |
NFκB (sc-8008) | Mono | Santa Cruz | NFκB, raised against amino acids 1–286 of NFκB p65 of human origin | 1 : 1,000 |
pNFκB (#3033) | Mono | Cell Signaling | phospho NFκB; raised against a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65 | 1 : 1,000 |
JNK (#9252) | Poly | Cell Signaling | SAPK/JNK, raised against a GST/human JNK2 fusion protein | 1 : 1,000 |
pJNK (#9251) | Poly | Cell Signaling | phospho-SAPK/JNK (Thr183/Tyr185), raised against a synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK | 1 : 1,000 |
pJNK (#4668) | Mono (r) | Cell Signaling | phospho-SAPK/JNK (Thr183/Tyr185) (81E11), raised again a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK. | 1 : 1,000 |
Casp-3 (#9664) | Mono (r) | Cell Signaling | cleaved Caspase-3 (Asp175) (5A1E), raised against a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3 | 1 : 1,000 |
LCN2 (AF3508) | Poly | R&D Systems, Wiesbaden, Germany | recombinant rat Lipocalin-2/NGAL produced in mouse myeloma cell line NSO, polyclonal goat IgG | 1 : 1,000 |
AIM-2 (14-6008-93) | Poly | eBioscience, San Diego, CA, USA | rabbit antibody that reacts with human, mouse, and rat AIM-2 | 1 : 1,000 |
NALP1 (4990) | Poly | Cell Signaling | human NALP1, raised against a synthetic peptide that corresponds to a region surrounding Gly1081 of human NALP1, crossreacts with human, mouse and rat NALP1 | 1 : 1,000 |
NALP3 (sc-66846) | Poly | Santa Cruz | Cryopyrin/NALP3 (H-66), rabbit antibody raised against amino acids 25–90 mapping near the N-terminus of human Cryopyrin | 1 : 1,500 |
NALP4/NLRP4 (ab 47241) | Poly | Abcam, Cambridge, UK | raised against synthetic peptide corresponding to amino acids 139–157 of human NALP4 | 1 : 1,000 |
β-actin (#A5441) | Mono | Sigma-Aldrich, Taufkirchen, Germany | β-actin, synthetic peptide N-terminus (clone AC-15) | 1 : 10,000 |
GAPDH (sc-32233) | Mono | Santa Cruz | GAPDH (clone 6C5) | 1 : 1,000 |
Secondary antibodies | ||||
sc-2004 | NA | Santa Cruz | goat anti-rabbit IgG-HRP | 1 : 5,000 |
sc-2056 | NA | Santa Cruz | donkey anti-goat IgG-HRP | 1 : 5,000 |
sc-2005 | NA | Santa Cruz | goat anti-mouse IgG-HRP | 1 : 5,000 |