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Table 2 Antibodies used in this study

From: Expression analysis of inflammasomes in experimental models of inflammatory and fibrotic liver disease

Primary antibodies
Protein/Antibody Clonality*    Supplied by Epitope, location Dilution
STAT1 (sc-346) Poly Santa Cruz Biotechnology, Santa Cruz, CA, USA raised peptide mapping near the C-terminus of STAT1 p84/p91 of human origin 1 : 500
pSTAT1 (#9167) Mono (r) Cell Signaling, Technology, Danvers, MA, USA phospho-STAT1 (Tyr701) (58D6), raised against synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human STAT1 1 : 1,000
STAT3 (#4904) Mono Cell Signaling raised a STAT3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3 protein 1 : 1,000
pSTAT3 (#9134) Poly Cell Signaling phospho-STAT3 (Ser727), raised against a synthetic phosphopeptide corresponding to residues surrounding Ser727 of mouse Stat3 1 : 1,000
NFκB (sc-8008) Mono Santa Cruz NFκB, raised against amino acids 1–286 of NFκB p65 of human origin 1 : 1,000
pNFκB (#3033) Mono Cell Signaling phospho NFκB; raised against a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65 1 : 1,000
JNK (#9252) Poly Cell Signaling SAPK/JNK, raised against a GST/human JNK2 fusion protein 1 : 1,000
pJNK (#9251) Poly Cell Signaling phospho-SAPK/JNK (Thr183/Tyr185), raised against a synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK 1 : 1,000
pJNK (#4668) Mono (r) Cell Signaling phospho-SAPK/JNK (Thr183/Tyr185) (81E11), raised again a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK. 1 : 1,000
Casp-3 (#9664) Mono (r) Cell Signaling cleaved Caspase-3 (Asp175) (5A1E), raised against a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3 1 : 1,000
LCN2 (AF3508) Poly R&D Systems, Wiesbaden, Germany recombinant rat Lipocalin-2/NGAL produced in mouse myeloma cell line NSO, polyclonal goat IgG 1 : 1,000
AIM-2 (14-6008-93) Poly eBioscience, San Diego, CA, USA rabbit antibody that reacts with human, mouse, and rat AIM-2 1 : 1,000
NALP1 (4990) Poly Cell Signaling human NALP1, raised against a synthetic peptide that corresponds to a region surrounding Gly1081 of human NALP1, crossreacts with human, mouse and rat NALP1 1 : 1,000
NALP3 (sc-66846) Poly Santa Cruz Cryopyrin/NALP3 (H-66), rabbit antibody raised against amino acids 25–90 mapping near the N-terminus of human Cryopyrin 1 : 1,500
NALP4/NLRP4 (ab 47241) Poly Abcam, Cambridge, UK raised against synthetic peptide corresponding to amino acids 139–157 of human NALP4 1 : 1,000
β-actin (#A5441) Mono Sigma-Aldrich, Taufkirchen, Germany β-actin, synthetic peptide N-terminus (clone AC-15) 1 : 10,000
GAPDH (sc-32233) Mono Santa Cruz GAPDH (clone 6C5) 1 : 1,000
Secondary antibodies
sc-2004 NA Santa Cruz goat anti-rabbit IgG-HRP 1 : 5,000
sc-2056 NA Santa Cruz donkey anti-goat IgG-HRP 1 : 5,000
sc-2005 NA Santa Cruz goat anti-mouse IgG-HRP 1 : 5,000
  1. * Abbreviations used for clonality are: Mono, monoclonal antibody produced in mouse; Mono (r), monoclonal antibody produced in rat; NA, not applicable; Poly, polyclonal antibody produced in rabbit.