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Table 1 Primers used for quantitative TaqMan analysis

From: Expression analysis of inflammasomes in experimental models of inflammatory and fibrotic liver disease

Rat

Gene of interest

GenBank no.

    Primers

NLRP-1

NM_001145755

For: 5’-gccctggagacaaagaatcc-3’

Rev: 5’-agtgggcatcgtcatgtgt-3’

NLRP-3

NM_001191642

For: 5’-gctgtgtgaggcactccag-3’

Rev: 5’-gaaacagcattgatgggtca-3’

NLRC-4

NM_001106707

For: 5’-ggccggaagtgaagctcta-3’

Rev: 5’-cccctccagttgcttcag-3’

AIM-2

XM_222949

For: 5’-tggaaaccagagcaaaacaa-3’

Rev: 5’-tgggctttgcagccttaata-3’

IL-1β

NM_031512

For: 5’-tgtgatgaaagacggcacac-3’

Rev: 5’-cttcttctttgggtattgtttgg-3’

IL-18

NM_019165

For: 5’-cctgatatcgaccgaacagc-3’

Rev: 5’-ccttccatccttcacagatagg-3’

ASC

NM_172322

For: 5’-gctcacaatgtctgtgcttagag-3’

Rev: 5’-gcagtagccacagctccag-3’

TNF-α

NM_012675

For: 5’-gcccagaccctcacactc-3’

Rev: 5’-ccactccagctgctcctct-3’

rS6

NM_017160

For: 5’-tgctcttggtgaagagtgga-3’

Rev: 5’-caagaatgccccttactcaaa-3’

Mouse

Gene of interest

GenBank no.

    Primers

NLRP-1a

NM_001004142

For: 5’-attttgtggccctccaaga-3’

Rev: 5’-ttgaaagtgggcaacatgg-3’

NLRP-1*

NM_001004142

For: 5’-tggcacatcctagggaaatc-3’

Rev: 5’-tcctcacgtgacagcagaac-3’

NLRP-3

NM_145827

For: 5’-cccttggagacacaggactc-3’

Rev: 5’-gaggctgcagttgtctaattcc-3’

NLRC-4

NM_001033367

For: 5’-tgatctccaagagatgaagttgg-3’

Rev: 5’-gatcaaattgtgaagattctgtgc-3’

AIM-2

NM_001013779

For: 5’-tcaggaagttttcctttttctca-3’

Rev: 5’-acagtcccaggatcagccta-3’

IL-1β

NM_008361

For: 5’-tgtaatgaaagacggcacacc-3’

Rev: 5’-tcttctttgggtattgcttgg-3’

IL-18

NM_008360

For: 5’-caaaccttccaaatcacttcct-3’

Rev: 5’-tccttgaagttgacgcaaga-3’

ASC

NM_023258

For: 5’-gagcagctgcaaacgactaa-3’

Rev: 5’-gtccacaaagtgtcctgttctg-3’

TNF-α

NM_013693

For: 5’-tcttctcattcctgcttgtgg-3’

Rev: 5’-ggtctgggccatagaactga-3’

IL-6

NM_031168

For: 5’-gctaccaaactggatataatcagga-3’

Rev: 5’-ccaggtagctatggtactccagaa-3’

IL-10

NM_010548

For: 5’-ggctgaggcgctgtcatcg-3’

Rev: 5’-tcattcatggccttgtagacacc-3’

IFN-γ

NM_008337

For: 5’-ggaggaactggcaaaaggatgg-3’

Rev: 5’-tgttgctgatggcctgattgtc-3’

CCL-2

NM_011333

For: gtgttggctcagccagatgc-3’

Rev: gacacctgctgctggtgatcc-3’

GAPDH

XM_001473623

For: 5’-actgccacccagaagactg-3’

Rev: 5’-caccaccctgttgctgtag-3’

rS6

BC092050

For: 5’-cccatgaagcaaggtgttct-3’

 

Rev: 5’-acaatgcatccacgaacaga-3’

 
  1. Note: * The primer combination for was taken from Ganz et al., WJG 2001;21:4772–8. It was taken to amplify NLRP-1 in hepatocytes. The NLRP-1a primer combinations were taken when NLRP-1 was amplified from total liver extracts.