Skip to main content

Table 1 Primers used for quantitative TaqMan analysis

From: Expression analysis of inflammasomes in experimental models of inflammatory and fibrotic liver disease

Rat
Gene of interest GenBank no.     Primers
NLRP-1 NM_001145755 For: 5’-gccctggagacaaagaatcc-3’
Rev: 5’-agtgggcatcgtcatgtgt-3’
NLRP-3 NM_001191642 For: 5’-gctgtgtgaggcactccag-3’
Rev: 5’-gaaacagcattgatgggtca-3’
NLRC-4 NM_001106707 For: 5’-ggccggaagtgaagctcta-3’
Rev: 5’-cccctccagttgcttcag-3’
AIM-2 XM_222949 For: 5’-tggaaaccagagcaaaacaa-3’
Rev: 5’-tgggctttgcagccttaata-3’
IL-1β NM_031512 For: 5’-tgtgatgaaagacggcacac-3’
Rev: 5’-cttcttctttgggtattgtttgg-3’
IL-18 NM_019165 For: 5’-cctgatatcgaccgaacagc-3’
Rev: 5’-ccttccatccttcacagatagg-3’
ASC NM_172322 For: 5’-gctcacaatgtctgtgcttagag-3’
Rev: 5’-gcagtagccacagctccag-3’
TNF-α NM_012675 For: 5’-gcccagaccctcacactc-3’
Rev: 5’-ccactccagctgctcctct-3’
rS6 NM_017160 For: 5’-tgctcttggtgaagagtgga-3’
Rev: 5’-caagaatgccccttactcaaa-3’
Mouse
Gene of interest GenBank no.     Primers
NLRP-1a NM_001004142 For: 5’-attttgtggccctccaaga-3’
Rev: 5’-ttgaaagtgggcaacatgg-3’
NLRP-1* NM_001004142 For: 5’-tggcacatcctagggaaatc-3’
Rev: 5’-tcctcacgtgacagcagaac-3’
NLRP-3 NM_145827 For: 5’-cccttggagacacaggactc-3’
Rev: 5’-gaggctgcagttgtctaattcc-3’
NLRC-4 NM_001033367 For: 5’-tgatctccaagagatgaagttgg-3’
Rev: 5’-gatcaaattgtgaagattctgtgc-3’
AIM-2 NM_001013779 For: 5’-tcaggaagttttcctttttctca-3’
Rev: 5’-acagtcccaggatcagccta-3’
IL-1β NM_008361 For: 5’-tgtaatgaaagacggcacacc-3’
Rev: 5’-tcttctttgggtattgcttgg-3’
IL-18 NM_008360 For: 5’-caaaccttccaaatcacttcct-3’
Rev: 5’-tccttgaagttgacgcaaga-3’
ASC NM_023258 For: 5’-gagcagctgcaaacgactaa-3’
Rev: 5’-gtccacaaagtgtcctgttctg-3’
TNF-α NM_013693 For: 5’-tcttctcattcctgcttgtgg-3’
Rev: 5’-ggtctgggccatagaactga-3’
IL-6 NM_031168 For: 5’-gctaccaaactggatataatcagga-3’
Rev: 5’-ccaggtagctatggtactccagaa-3’
IL-10 NM_010548 For: 5’-ggctgaggcgctgtcatcg-3’
Rev: 5’-tcattcatggccttgtagacacc-3’
IFN-γ NM_008337 For: 5’-ggaggaactggcaaaaggatgg-3’
Rev: 5’-tgttgctgatggcctgattgtc-3’
CCL-2 NM_011333 For: gtgttggctcagccagatgc-3’
Rev: gacacctgctgctggtgatcc-3’
GAPDH XM_001473623 For: 5’-actgccacccagaagactg-3’
Rev: 5’-caccaccctgttgctgtag-3’
rS6 BC092050 For: 5’-cccatgaagcaaggtgttct-3’
  Rev: 5’-acaatgcatccacgaacaga-3’  
  1. Note: * The primer combination for was taken from Ganz et al., WJG 2001;21:4772–8. It was taken to amplify NLRP-1 in hepatocytes. The NLRP-1a primer combinations were taken when NLRP-1 was amplified from total liver extracts.