Figure 4
Expression of inflammasome components in rat livers after application of CCl 4 . (A) Sprague Dawley rats received one single application of CCl4 or oil. 48 hrs later RNA was prepared and analyzed for expression of IL-1α, IL-1β, IL-6, IL-10, IL-18, IFN-γ, ASC, TNF-α, NLRP-1, NLRP-3, NLRC-4 and AIM2 by qRT-PCR. Details on primer combinations are given in Table 1. (B) The same set of RNAs from (A) was analyzed for expression of CCL-2. (C) The livers of Sprague Dawley rats (3 animals/group) that received one single (1x) or twice weekly intraperitoneal injections of 1 ml/kg BW of CCl4 in an equal volume of mineral oil for 1, 2, 4, 8, or 12 weeks were sampled, RNA extracted, and the expression of NLRP-3 (C), NLRP-1 (D), and AIM2 (E) analyzed by qRT-PCR. Representative melting curves for the expression studies of the three genes investigated are depicted in the lower panels. For detailed information about primer combinations and statistical analysis of this set of experiments see Table 1 and Additional file 1.