Figure 2

Expression of TLRs’ adaptor proteins in PBMCs. The PBMCs from 4 individuals in each group (lean, overweight and obese) were used for total RNA isolation. MyD88, IRAK1 and TRAF6 mRNA were measured by RT-PCR (A). The RNA expression was normalized to GAPDH housekeeping gene expression. MyD88, IRAK1 and TRAF6 mRNA expression is shown in arbitrary units for each group. Results are expressed relative to the expression level in controls and are represented as means ± SE values (B, C and D). A strong association of MyD88 and IRAK1 with BMI was observed in PBMCs (E and F).