Figure 1

Expression of TLR2 and TLR4 mRNA in PBMCs. The PBMCs from 4 individuals in each group (lean, overweight and obese) were used for total RNA isolation. TLR2 and TLR4 mRNA were measured by RT-PCR. Jurkat cells do not express TLR2 and TLR4 (−ve control). THP1 cells express TLR2 and TLR4 (+ve control) (A). The RNA expression was normalized to GAPDH housekeeping gene expression. TLR2 and TLR4 mRNA expression is shown in arbitrary units for each group. Results are expressed relative to the expression level in controls and are represented as means ± SE values (B and C). A strong association of TLR2/ TLR4 mRNA with BMI was observed in PBMCs (D and E).