Relative gene expression of iNOS (A) and eNOS (B) in pAECs treated with LPS (10 μg/ml) at different time periods (n = 3). LPS increased iNOS mRNA expression while eNOS mRNA expression was decreased. Relative mRNA data are expressed as the fold of increase (ΔΔCt method) in respect to the control (CTR = mean ± SEM of all control time points). Error bars represent the range of relative expression. The different letters above the bars indicate significant differences in the various time points (p < 0.05, ANOVA post hoc Tukey’s test).