Figure 2

CD147 expression in the synovial tissues of CIA mice. (a) Representative western blots of CD147 levels in tissue lysates of the synovial joints of control and CIA mice. GAPDH was used as an internal control. Densitometric analysis was performed on protein bands and the data are expressed as the percentage of control values. Three separate experiments (n = 3 mice in each) were performed (control: 100.0 ± 17.4%, CIA: 1144.2 ± 125.1%). (b) Representative photographs showing immunohistochemical staining of CD147 in synovial tissues of control and CIA mice (left and middle panel, respectively; n = 4 mice in each group). Right panel (negative control) showing immunohistochemical staining of CD147 in synovial tissues of CIA mice according to the method described above, except that the primary antibody was omitted. (c) Representative photographs showing immunohistochemical staining of CD147 in infiltrated CD11b-immunopositive cells (mainly for macrophages) of synovial tissues of control and CIA mice (top and middle panel, respectively; n = 4 mice in each group). Frozen joints sections were stained with antibodies for CD11b (green) and CD147 (red) for double immunofluorescence staining. Bottom panel (negative control) showing double immunofluorescence staining of CD11b and CD147 in synovial tissues of CIA mice according to the method described above, except that the primary antibodies were omitted.