Figure 3

Reduced foam cell formation in human CRP transgenic mice may be explained by CRP inhibition of the association of oxLDL with macrophages. Human macrophages were incubated with 25 μg/ml of DiI-oxLDL for 30 min with or without hCRP as indicated. In a subset experiments, FcγRI (CD64), FcγRII (CD32) or CD36 was pre-blocked by incubating with 15 μg/mL monoclonal IgGs for 15 min prior to incubation with DiI-oxLDL. The data represent mean ± SD of specific cell-associated fluorescence from three independent experiments. Significance was determined using an ANOVA followed by unpaired t tests. *; p < 0.05 vs. the control (#), NS; not statistically significant.