Figure 7

Splenic macrophages, DCs, and NK cells, and colon F4/80⁺cells were reduced in H . bilis -infected Rag2^−/−mice treated with anti-IL-7Rα M595. Spleens were harvested from mice and cells were stained with antibodies to identify myeloid-cell subsets by flow cytometry. (A) Absolute numbers of splenic myeloid cells were determined using Advia Hematology Analyzer. (B) Flow cytometry gating strategy for splenic DC- and macrophage-cell populations using CD11c and CD11b staining. (C) Absolute numbers of splenic pDC, mDC, NK cells (gated on DX5⁺ cells), and macrophages, along with corresponding absolute numbers of IL-7R⁺ pDC, mDC, NK cells, and macrophages, were derived from flow cytometry percentages and Advia myeloid-cell counts. (D) Sections of colon and cecum were immunostained with F4/80. The F4/80 score is a ratio of the number of F4/80⁺ cells to total section area. ANOVA followed by Dunnett’s or non-parametric t-test for all groups vs. isotype Hb. *p < 0.05, **p < 0.001, ***p <0.0001.