Figure 6

Treatment with anti-IL-7Rα repressed IBD in T and B-cell deficient Rag2^−/−mice. Seven to eleven-week-old Rag2^−/− mice were treated IP twice weekly with 250 μg or 50 μg of anti-IL-7Rα M595, or 250 μg of isotype control antibody one week prior to oral gavage with Hb (1×10⁶ CFU administered twice, on 2 consecutive days) or broth and then treated twice weekly throughout the duration of the study for 11 weeks post-infection. (A) Histopathological IBD scores from colon. Cecum and colon IBD scores were based on severity of mucosal epithelial changes, degree of inflammation and extent of lesions [11]. (B) Percent change in weight is shown for mice 1 week prior to infection through 10 weeks post infection. (C) Representative H&E-stained sections of proximal colon from uninfected control (isotype broth), infected control (isotype Hb) and anti-IL-7Rα M595 treated (250 μg) Hb-infected mice. Original magnification, 20X. ANOVA followed by Tukey's post-test on square root transformed data (a). *p < 0.05, **p < 0.001, ***p <0.0001.