Messenger RNA expression in H . bilis-infected Mdr1a−/−mice were altered with anti-IL-7Rα M595 treatment. Heat maps show hierarchical clustering of expression of colon mRNA (A) coding fold changes of the indicated comparisons. (A) RNA was extracted from 5 mm of proximal colon from a subset of mice (for isotype Hb group, two mice with lower scores and four with higher scores were analyzed). RNA was converted to cDNA and then analyzed using TaqMan® Mouse Immune Array. (B) Expression of proximal colon CD4 mRNA (relative to HPRT) from the immune array, and (C) proximal colon IL-7Rα mRNA (relative to GAPDH) from single-gene RT-PCR, is shown for individual mice in each treatment group. For (A), genes were filtered to show only those that were significantly (p < 0.05) altered between isotype Hb and isotype-broth-treatment groups. Red indicates an increase in expression and green indicates a decrease in expression. For (B) and (C), ANOVA followed by multivariate t method. *p < 0.05, **p < 0.001, ***p < 0.0001.