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Table 2 LPS-dependent changes in ornithine derived products

From: A new metabolomic assay to examine inflammation and redox pathways following LPS challenge

   

LPS Concentrations

  

Metabolite

Control

0.1 ng/ml

1 ng/ml

10 ng/ml

100 ng/ml

1000 ng/ml

Orn

0.78 ± 0.20

1.07 ± 0.25

1.36 ± 0.31

1.47 ± 0.88

3.26 ± 0.24*

5.86 ± 0.83**

Put

1.21 ± 0.39

2.88 ± 0.98

6.05 ± 2.8**

7.94 ± 3.11**

9.56 ± 4.28**

9.31 ± 3.42**

Pro

5.45 ± 1.62

6.77 ± 1.36

7.81 ± 2.90

7.38 ± 1.98

7.07 ± 1.82

6.04 ± 1.33

Spd

13.32 ± 2.09

15.72 ± 2.28

13.67 ± 2.35

13.93 ± 2.09

13.66 ± 1.60

13.74 ± 2.38

Spm

23.82 ± 1.55

14.07 ± 3.05*

12.60 ± 2.48*

10.69 ± 2.75**

7.39 ± 1.86**

6.75 ± 2.04**

  1. RAW cells (2 × 106) cells were incubated in the absence (control) or in the presence of 0.1, 1, 10, 100 and 1000 ng/ml LPS (LPS) for 24 hrs. Cellular metabolites were extracted and analyzed as described in methods. Results are an average of 3 independent experiments. * and ** denotes p < 0.05 or p < 0.01, respectively.
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Journal of Inflammation

ISSN: 1476-9255

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