Figure 2
From: A new metabolomic assay to examine inflammation and redox pathways following LPS challenge
NO formation in LPS-activated macrophage is regulated by changes in iNOS and Arginase activities. The concentration dependent effects of LPS on intracellular Arg (panel A) and its metabolic products, Cit (panel B) and Orn (panel D) are shown. The Cit/Orn ratio, which indicates the balance between NOS and arginase dependent pathway is plotted in (panel E) and compared to the media total media nitrate/nitrite (NOx; panel C) concentration. Raw cells (2 × 106 cells) were either left untreated or incubated with 0.1, 1,10, 100 and 1000 ng/ml LPS at 37°C for 24 hrs.