Figure 4
From: NOD2 is highly expressed in Behçet disease with pulmonary manifestations
Comparative analysis of NOD1 and NOD2 mRNA expression in peripheral blood mononuclear cells (PBMC) obtained from 10 active Behcet disease (BD) patients with pulmonary manifestations before and after treatment after their symptoms had resolved. NOD1 and NOD2 mRNA expression was analysed, by real-time RT-PCR, in peripheral blood cells isolated from BD patients and matched controls. To permit comparison between individuals, absolute copy numbers of NOD1 and NOD2 mRNA were measured during RT-PCR, using cDNA standards, and expressed relative to 106 mRNA copies of a validated housekeeping gene HuPO. NOD2 in 3/10 patients but not NOD1 mRNA levels increased significantly after treatment. Statistical significance was evaluated by the Wilcoxon test