Figure 1

NOD1 and NOD2 mRNA expression in BAL leucocytes obtained from Behçet's disease (BD) patients. [A]: NOD1 and NOD2 mRNA expression in BAL from BD and sarcoidosis patients compared to healthy controls. NOD1 and NOD2 mRNA expression were measured by real-time RT-PCR. To permit comparison between individuals, absolute copy numbers of NOD1 and NOD2 mRNA were measured during RT-PCR, using cDNA standards, and expressed relative to 10⁶ mRNA copies of a validated housekeeping gene, HuPO. [B]: Detection of NOD1 and NOD2 protein in bronchoalveolar lavage cells from BD patients by flow cytometry. Histograms shown are representative of two independent experiments. Staining against NOD1 and NOD2 (open histogram), appropriate isotype controls (light shaded histogram) or secondary Abs (dark shaded histogram). Data show one representative out of three independent experiments