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Table 2 Primers/Probes used for real-time RT-PCR (TaqMan® Gene Expression Assays, Applied Biosystems)

From: Curcuma DMSO extracts and curcumin exhibit an anti-inflammatory and anti-catabolic effect on human intervertebral disc cells, possibly by influencing TLR2 expression and JNK activity

Gene

Primer sequence number

Base pairs

Interleukin-1β (IL-1β)

Hs00174097_m1

94

Interleukin-6 (IL-6)

Hs00174131_m1

95

Interleukin-8 (IL-8)

Hs00174103_m1

101

Matrixmetalloproteinase-1 (MMP1)

Hs00233958_m1

133

Matrixmetalloproteinase-3 (MMP3)

Hs00968308_m1

98

Matrixmetalloproteinase-13 (MMP13)

Hs00233992_m1

91

TATA box binding protein (TBP)

Hs00427620_m1

91

Toll-like receptor 2 (TLR2)

Hs00152932_m1

80

Tumor necrosis factor alpha (TNF-α)

Hs00174128_m1

80

  1. Data was obtained by real-time RT-PCR (ΔΔct method) (n = 10).