Effects of curcumin on nuclear translocation of p65/NF-κB, measured by immunoblotting of p65 (n = 3, nuclear extracts, 60 min). 6a: Nuclear translocation of p65, measured by immunoblotting (n=3, nuclear extracts, 60 min). One representative sample is shown and PARP-1/2 is used as a loading control. 6b: NF-kB DNA binding activity, measured by NF-kB transcription factor assay (n=2, nuclear extracts, 60 min: without statistical evaluation). Note that different internal controls (positive control, competition control and unspecific binding control) were used to validate the test.