Table 2 Primer sequences used for quantitative Real-time RT-PCR
| Gene | Primer pair Sequence (5′-3′) | Annealing Temperature |
|---|---|---|
| hTERT | FOR: TGACACCTCACCTCACCCAC | 51°C |
| REV: CACTGTCTTCCGCAAGTTCAC | ||
| hTERC | FOR: GCCTGCCGCCTTCCACCGTTCATT | 59°C |
| REV: GACTCGCTCCGTTCCTCTTCCTG | ||
| TELOMERE | FOR: CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT | 56°C |
| REV: GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT | ||
| ACTIN | FOR: CGGCATCGTCACCAACTG | 58°C |
| REV: GGCACACGCAGCTCATTG |