Figure 4

Expression levels of oxidative index and anti-oxidant protein in infarcted area. A & B) The protein expression of oxidative stress (n = 6). Oxidative index, protein carbonyls, was substantially higher in AMI than in normal and AMI + tacrolimus, and remarkably higher in AMI + tacrolimus than in normal. * vs. other groups, p < 0.0001. C & D) Expression levels of anti-oxidant proteins (n = 6). Protein expression of heme oxygenase (HO)-1 (C) and NAD(P)H:quinone oxidoreductase (NQO)-1 (D) were notably higher in AMI + tacrolimus than in AMI and normal, and significantly higher in AMI than in normal. * vs. other groups, p < 0.005. All statistical analyses using one-way ANOVA, followed by Tukey’s multiple comparison procedure. Symbols (*, †, ‡) indicate significance (at 0.05 level).