Figure 4

a, b, c, d, e, f. Decreased complement activity in blood serum of Crry^−/−EAMG mice did not alter the amount of complement deposition at the NMJs. Diaphragms from WT EAMG (Figure4a, b, c) and Crry ^−/− EAMG (Figure4d, e, f) mice were stained for the presence of C3, C3b/iC3b and C5b-9 (green fluorescence) complement components. The NMJs were visualized with bungarotoxin (BTX; red fluorescence). Not immunized WT Ctrl and Crry ^−/− Ctrl mice did not show complement deposition at the NMJ (data not shown). Diaphragms of a minimum of 5 mice from each experimental group were analyzed. Scale bars for magnification (20 μm) are shown on each microphotograph. See Material and Methods for experimental details.