Figure 3From: Activation of human neutrophils by Esenbeckia leiocarpa: comparison between the crude hydroalcoholic extract (CHE) and an alkaloid (Alk) fractionCHE and Alk enhance neutrophil adhesion onto cell substratum. PMNs were incubated with or without CHE, Alk, TNF-α, CHE + TNF-α or Alk + TNF-α for 30 min, labelled with calcein-AM, incubated on confluent A549 cells for 30 min and adhesion was measured as described in Methods. Results are means ± SEM (n = 4) and are expressed as indices, one being the basal or control level.Back to article page