Figure 1
Effect of CHE and Alk on intracellular ROS production in human neutrophils. Freshly isolated human PMNs (1 x 106 cells/mL) were incubated with diluent (1% DMSO in HBSS, Ctrl), the positive control, PMA, CHE (500 μg/mL), Alk (100 μg/mL), PMA + CHE or PMA + Alk for 5, 15 or 30 min and intracellular ROS production was assessed by flow cytometry as described in Materials. Results are means ± SEM (n = 4). *, p < 0.05 vs Ctrl; #, p < 0.05 vs PMA by student-t test.