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Table 3 Immunofluorescence staining for markers of complement activation

From: Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgery

Antibody and complement deposition in tissue

Baseline

End ischemia

10 min reperfusion

P value

IgG

1.03 ± 9.60*

0.81 ± 0.60

0.50 ± 0.40

n.s.

IgM

0.02 ± 0.02

0.06 ± 0.08

0.03 ± 0.03

n.s.

C3b/c

0.12 ± 0.13

0.12 ± 0.22

0.13 ± 0.22

n.s.

C4b/c

0.16 ± 0.10

0.29 ± 0.46

0.22 ± 0.31

n.s.

  1. *Values are mean ± standard deviation of immunofluorescence scores (quantitation by Imaris software), n = 10.