Immunofluorescence staining for HSPG. Tissue samples from baseline, end ischemia and 10 min reperfusion were analyzed by Immunofluorescence for HSPG. Pictures are representative for the shown time points. No statistical differences in HSPG expression were found by Repeated Measures ANOVA, n = 10. Green = HSPG, blue = DAPI staining of nuclei. Scale bars = 150 μm.