Figure 4

CD11b + Gr-1+ cells from spleens of HV-68 infected animals harbor HV-68 viral genomes. Groups of Balb/c mice were mock-treated (M) or intranasally infected with HV-68 virus (HV-68). During the peak of viral-induced leukocytosis (15 days post-infection), bone marrow cells and splenocytes were isolated from individual animals and unfractionated (Spleen, Bone Marrow) or fractionated into CD11b positive (CD11b+), CD11b negative (CD11b-), or CD11b positive plus Gr1 positive (CDb + Gr-1+) cell populations. DNA was isolated from each cell fraction and assayed for the presence of viral ORF65 by PCR. Representative results of one set of cell fractionations (N = 3) are presented as amplified products electrophoresed on ethidium bromide-stained gels. The presence of the Taar1 gene in each DNA sample using PCR was also performed as a control for DNA loading in each sample