Figure 3
From: Murine gammaherpesvirus-68 expands, but does not activate, CD11b+ gr-1+ splenocytes in vivo
Fractionation of CD11b + Gr1+ spleen cells into Ly-6 G + and Ly-6 G- populations. Groups of Balb/c mice were intranasally infected with HV-68 virus, and at 15 days post-infection splenocytes were isolated from individual animals. CD11b + Gr1+ cells were isolated from these splenocytes, and labeled with fluorchrome-conjugated anti-Ly-6 G and anti-Ly-6 C antibodies. FACS analysis was performed to determine the percentage of each cell population. Results are presented as mean values (N = 5) ± standard errors. These studies were performed three times with similar results.