Figure 4From: Microglia differentiation using a culture system for the expansion of mice non-adherent bone marrow stem cells(A) Representative scatter plots of differentiated cells. A microglia gate was set, defined by the primary microglia population, to assess differentiation. (B) Percent of CD11b/CD45low (microglia gate) among the differentiated cells (n=3). Yields of cells showing the microglia markers are not different between the two protocols. A small subpopulation in fresh bone marrow (10%) shows microglia markers from the beginning. (C) and (D) Percent of CD11b+ and CD45+ cells among the differentiated cells (n=3). CD45 is already high in fresh bone marrow. P1: Protocol 1. P2: Protocol 2. *** = P ≪ 0.001, ** = P ≪ 0.005, * = P ≪ 0.01.Back to article page