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Figure 2 | Journal of Inflammation

Figure 2

From: Microglia differentiation using a culture system for the expansion of mice non-adherent bone marrow stem cells

Figure 2

(A) Scatter plots of NA-BMC during the selective adhesion phase. The indicated regions define populations of immature and nucleated red blood cells, lymphocytes, monocytes and granulocytes. P is the probability of the null hypothesis of the linear regression, i. e. that the percentage fraction does not change over time. (B) Changes in the percentage fraction of the cell populations is shown. Lymphocyte fraction is diminished by selective adhesion. (C) CFU-f grown from NA-BMCs of day 1 to day 4 (n=3). Methylene blue staining was used to determine total colonies. Alizarin red (Calcium), Sirius red (Collagen) and alkaline phosphatase (ALP) staining were used to detect the capacity of NA-BMC for osteogenic differentiation.

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