Figure 6

Inhibition of LPS-induced pAkt by the G-protein inhibitor pertussis toxin and the NOX inhibitor Apocynin. PBMCs from healthy volunteers were pretreated with either Apocynin (300 μM) or pertussis toxin (100 ng/ml) for 30 min before LPS stimulation. Phosphorylated AKT and total AKT levels were visualised by Western blot and assessed semi-quantitatively by band densitometry. The data was normalised to total AKT for each treatment group and plotted as mean ± S.E.M of 7 independent experiments (**p < 0.01 as determined by Kruskal Wallace non-parametric ANOVA followed by Dunnets post-test analysis) showing the fold-increase in phospho-Akt compared to unstimulated cells.