IL-1β, IL1RN, TNF-α and TACE genes are differentially upregulated in both adipose and liver tissues of HFC-fed mice. Expression levels of IL-1β, IL1RN, TNF-α and TACE genes from chow (in black) and HFC (in red) fed mice at each time point are presented as average ∆Ct of all animals in each group (details in Methods). The smaller ∆Ct value indicates the higher expression level. EF stands for epididymal fat pad and MF for mesenteric fat pad. The MF sample of 6 week/Chow and the liver samples of 6 week/Chow and 6 week/HFC had no signal for IL1RN because of very low expression level. In addition, the fold change of mRNA levels in HFC group vs. chow group is also presented below the expression level panel for each gene. Statistical significance was determined by two-tailed Welch t test where P < 0.05 (*), P < 0.01 (**), and P < 0.001 (***).