Figure 3

SIN-1, a NO donor shows similar patterns on viability and NO accumulation in microglia as LPS treatment. BV2 cells were incubated in increasing doses of SIN-1 for 24 hr. NO accumulation as determined by the Greiss reagent (A) increased in a dose dependent manner. Viability, as assessed by light microscopy and MTT quantification, also decreased, but only at concentrations of 0.5 mM or greater. n = 6 independent observations, *P < 0.05 versus control