Figure 4

Stimulated PBMCs derived from subjects on OMD and TMD diets were examined for IL-17 production and for mRNA expression of IL-17R and IL-23. PBMCs isolated from subjects at the indicated time points were stimulated with anti-CD3 mAb antibody. (A.) The IL-17 secretion at 1 month time point was significantly lower in OMD fed subjects compared to TMD (p < 0.05). The data are expressed in pg/ml (+/- SEM). (B.) Equal amounts of cDNA from individual donors were analyzed for IL-17R and IL-23 mRNA levels using real-time RT-PCR. Each sample was run in duplicate and the threshold value (Ct) was normalized to GAPDH and is expressed as average fold change. The numbers of subjects (n) examined for each stage of the study are as follows: Pre-treatment/Baseline (n = 15), 1 meal/1 month (n = 8), 1 meal/2 month (n = 12), Off-diet (n = 12), 3 meals/1 month (n = 12) and 3 meal/2 month (n = 12).