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Figure 3 | Journal of Inflammation

Figure 3

From: Controlled meal frequency without caloric restriction alters peripheral blood mononuclear cell cytokine production

Figure 3

Stimulated PBMCs derived from subjects on OMD and TMD diets were examined for TNF-α and IFN-γ expression. Peripheral blood mononuclear cells derived from OMD and TMD diets were stimulated ex-vivo with anti-CD3 mAb or LPS. In both the study groups, there was a statistically significant (p < 0.05) increase in TNF-α and levels in culture supernatants at one month after initiation of dietary regimens. (A) LPS-induced TNF-α release at 1 month time point was significantly lower in OMD fed subjects compared to the TMD group. No significant differences could be detected at other time points and in response to anti-CD3 mAb stimulation. (B) T cell activation by TCR-dependent mechanisms (anti-CD3 mAb) results in a lower IFN-γ release at one month time point in subjects fed OMD versus those fed TMD. The data are expressed in pg/ml (+/- SEM). The numbers of subjects (n) examined for each stage of the study are as follows: Pre-treatment/Baseline (n = 15), 1 meal/1 month (n = 8), 1 meal/2 month (n = 12), Off-diet (n = 12), 3 meals/1 month (n = 12) and 3 meal/2 month (n = 12).

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