Figure 5

Establishment of angiogenesis: Figure 5A demonstrates VEGF-A was significantly up- regulated at 2 hours after wounding in the knockout group compared to the control group. At day 7 after wounding, VEGF-A expression between test and control groups was significantly different, with VEGF-A expression in the knockout group returning to baseline levels, and in the control group appearing to have increased. Figure 5B demonstrates CD31⁺ cell recruitment into wound sites. The number of CD31⁺ cells recruited per high-power microscopic fields counted. Data is expressed as the mean ± SEM (n = 3). There was a higher number of CD31⁺ cells observed 1 day after wounding in the knockout mice compared to control group. This was not statistically significant. There was a statistically significant difference in the number of CD31⁺ cells at day 4 after wounding between test and control group (t-test; p value 0.002).