Zinc gluconate decreased airway inflammation in vivo. Naïve BALB/c mice were given an i.p. injection of PBS or zinc gluconate (0.1, 1, or 10 mg/kg) once a day for 3 days prior to a single i.t. inhalation of GC frass (40 μg/40 μl). 18 h later, BAL fluid was harvested, infiltrated cells counted and BAL fluid was analyzed by ELISA. In all cases means ± SEM are shown (n = 6 mice per group). A. Total cells in BAL fluid (different from PBS *p < 0.001; different from GC frass treated **p < 0.05). B. TNFα ELISA (different from PBS * < 0.001; different from GC frass treated without zinc supplementation **p < 0.05, as determined by ANOVA).