Figure 8

SR141716A increases adiponectin gene expression, but not secretion, and has no effect on A-FABP gene expression in differentiated pre-adipocytes. Panel A: A-FABP gene expression was determined at 10 days in differentiated pre-adipocytes cultures, treated or not with SR141716A (200 and 500 nM). The graph shows the mean ± SD of the results from 2 patients (n = 6 for each condition, for each patient). Panel B: Adiponectin gene expression was determined at 10 days in differentiated pre-adipocytes cultures, treated or not with SR141716A (200 and 500 nM). The graph shows the mean ± SD of the results from 3 patients (n = 6 for each condition, for each patient). **P < 0.005, versus control cells. Panel C: Differentiated SVF cells were treated or not with SR141716A (100 nM and 200 nM) for 48 hours. The medium was assayed for adiponectin using ELISA. The graph shows the mean ± SD of the results from 2 patients (n = 6 for each condition, for each patient).