Figure 2

SR141716A decreases LPS-induced IL-6 secretion but not gene expression in mature adipocytes. Panel A: Adipocytes were treated with 1 μg/mL LPS and/or not with SR141716A from 50 to 400 nM. IL-6 secretion was measured in media after 12 hours treatment by ELISA. Results are expressed in percentage, normalised to LPS (100% represents from 1 to 10 ng/mL IL6, depending on the patients). The graph shows the mean ± SD of the results from 3 patients (n = 6 for each condition, for each patient). ***P < 0.001, **P < 0.005 and *P < 0.05, versus LPS-treated cells. Panel B: IL6 gene expression was determined at 4 hours of treatment in mature adipocyte cultures, treated or not with LPS 1 μg/mL alone or in combination with 200 nM SR141716A. The graph shows the mean ± SD of the results from 2 patients (n = 6 for each condition, for each patient).